Computational Enzyme Design: Refining Artificial Enzymes and Exploring Paths of Directed Evolution

Directed evolution of industrial enzymes.

Directed evolution of glycosylating enzymes

s 54

Reprogramming homing endonuclease specificity through computational design and directed evolution

Homing endonucleases (HEs) can be used to induce targeted genome modification to reduce the fitness of pathogen vectors such as the malaria-transmitting Anopheles gambiae and to correct deleterious mutations in genetic diseases. We describe the creation of an extensive set of HE variants with novel DNA cleavage specificities using an integrated experimental and computational approach. Using com...

Directed evolution of enzymes and biosynthetic pathways.

Directed evolution is an important tool for overcoming the limitations of natural enzymes as biocatalysts. Recent advances have focused on applying directed evolution to a variety of enzymes, such as epoxide hydrolase, glyphosate N-acetyltransferase, xylanase and phosphotriesterase, in order to improve their activity, selectivity, stability and solubility. The focus has also shifted to manipula...

Directed Evolution: Novel and Improved Enzymes

• A Primer for Directed Evolution • Methods for Directed Evolution • Applications of Directed Evolution • Conclusions and Future Prospects Directed Evolution: Novel and Improved Enzymes Fei Wen∗, Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, Illinois Michael McLachlan∗, Center for Biophysics and Computational Biology, University of Illi...